February 16, 2024

“First, we confirmed the *in vitro* cytocompatibility by incubating sEV formulations (NR2B9c Conc, 25, 50, 100, 200 nM) with primary neurons.”

4:05 AM

“sEV cellular internalization was first studied in Neuro-2a cells”

4:07 AM

“RVG29 conjugation increased the cellular uptake characteristics of sEVs compared with naïve sEVs”

4:08 AM

“RVG29 is the only rabies virus protein that attaches to the *N*-acetyl choline receptor [[46](https://www.sciencedirect.com/science/article/pii/S0168365923006533?via%3Dihub=#bb0230)] abundantly present on neuron”

4:08 AM

“confirm the neuron targeting potential of RVG-sEVs”

4:08 AM

“click chemistry technique effectively conjugates RVG29 to sEVs surface, resulting in efficient sEV neurons targeting.”

4:09 AM

“Near-infrared [fluorescent dye](https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/fluorescent-dye), DiR labeled free sEVs, RVG-sEVs, and free DiR were i.v. injected to track sEVs *in vivo* and *ex vivo* in tMCAO mice using *In Vivo* Imaging system (IVIS).”

4:10 AM

“sEVs based brain targeted cargo delivery system”

4:52 AM

“delivery of neuroprotective peptide (NR2B9c) to the brain, overcoming BBB, targeting neurons, increased biodistribution, and bioavailability.”

4:53 AM

“We engineered sEVs with neuron-targeting peptide RVG29 *via* click reactions and used them for specific delivery of NR2B9c with higher efficiency to ischemic neurons for prevention of excessive ROS production during brain ischemia”

4:53 AM